Journal: Cell systems

Article Title: Geometry dependent arrhythmias in electrically excitable tissues

doi: 10.1016/j.cels.2018.08.013

Figure Lengend Snippet: Key Resources Table

Article Snippet: ​ REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, Peptides, and Recombinant Proteins Isradipine Tocris Bioscience 2004 Puromycin ThermoFisher A1113802 Blasticidin ThermoFisher A1113902 Fibronectin Yo Proteins Cat #663 Acrylic acid N-hydroxysuccinimide ester Sigma A8060 Acrylamide solution (40%) Sigma A4058-100ML Acrylamide/Bisacrylamide, (40% solution, 19:1) Sigma A9926-100ML SU-8 3025 Photoresist Microchem SU-8 3025 SU-8 Developer Microchem N/A 3-(Trimethoxysilyl)propyl methacrylate Sigma 2530-85-0 Anhydrous ethanol Sigma 277649-100ML Glacial acetic acid Sigma A6283-100ML Potassium persulfate Sigma 7727-21-1 Experimental Models: Cell Lines Optopatch-Spiking HEK Cells This paper, McNamara et al 2016 32 N/A iCell Cardiomyocytes Cellular Dynamics Kit 01434 Recombinant DNA pENTR-L5-Kir2.1-mCherry-L2 Addgene 32,669 pIRESpuro3-NaV1.5 Johns Hopkins University ChemCORE N/A FCK-Optopatch1 Hochbaum et al 2014 35, Addgene 51695 FCK-CMV-CheRiff-CFP N/A psPAX2 Addgene 12260 VsVg Addgene 12259 Software and Algorithms MATLAB code for image processing and data analysis This paper Supplement MATLAB code for iOS-HEK model simulation and dispersion analysis This paper Supplement MATLAB code for Noble model simulation This paper Supplement Other Firefly Widefield Microscope This paper, Werley et al 2017 39 N/A Silicon Wafers University Wafers 58.52-1 Lithography transparency mask CADArt Services N/A (custom order) Open in a separate window Key Resources Table An engineered excitable cell line becomes arrhythmic when rapidly paced.

Techniques: Recombinant, Software, Microscopy